Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Secondary antibody
Name
Dilution
1/500
Additional data
Additional Notes
I did not obtain any staining with this antibody. I tested it at different dilutions between 1:100-1:3000 using mouse brain sections. This antibody is not good for IHC using perfused-fixed sections.
Abcam response
Thank you for your honest ant thoughtful feedback. We have yet to test this antibody on perfused free-floating sections, and it may indeed be unsuitable for use in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
投稿 Oct 02 2008