Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton-X 100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+10% donkey serum 0.1% Triton X-100
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa flour 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
I tried 3 dilutions and 1/100, 1/500, 1/1000. The 1/100 gave strongest signal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Osama Aldalahmah
Verified customer
投稿 Aug 30 2013