Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 4°C
Sample
Human Cell (Human arterial myocytes)
Specification
Human arterial myocytes
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Physiological saline solution
Dilution
1/250
Secondary antibody
Name
Non-Abcam antibody was used: MFP 555 donkey anti-rabbit (Mobitec, Germany)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Dilution
1/400
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 08 2015