Application
Western blot
Sample
Human Cell lysate - nuclear (SW1353)
Loading amount
20 µg
Specification
SW1353
Treatment
50ng/mL rhWnt3A
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris Gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
8 second(s)
Bands
Specific: approx 98 kDa
Additional data
Additional Notes
1.) SW1353 cells treated with 50ng/ml of rhWnt3A for 24 hours.
2.) SW1353 cells over-expressed SOST treated with 50ng/mL of rhWnt3A for 24 hours
2.) SW1353 cells over-expressed SOST treated with 50ng/mL of rhWnt3A for 24 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 30 2013