Application
Western blot
Sample
Human Cell lysate - whole cell (human breast cancer cells)
Gel Running Conditions
Reduced Denaturing (Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl)
Loading amount
100 µg
Specification
human breast cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 23°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Concentration
0.25 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
HRP Vector VIP kit
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 26 kDa
Positive control
n/a
Additional data
Additional Notes
Two bands always appeared- had to order ab32572 to resolve the issue.
Abcam response
Thank you for submitting an AbReview and your honest feedback. We appreciate the time and effort that the reviewer has put in to testing this product and we are sorry to see that the antibody was not working as expected. The customer has received a vial of ab32572 free of charge in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Katie Elliott
Verified customer
投稿 Aug 01 2016