Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mammary epithelial cells)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization
No
Specification
Mammary epithelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% non-immune goat serum
Dilution
1/800
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 594 anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
Thank you for your valuable feedback about the use of this antibody in pig, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in pig which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Katie Elliott
Verified customer
投稿 Aug 15 2016