Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (mouse Embryonic stem cell)
Specification
mouse Embryonic stem cell
Permeabilization
Yes - 0.1% triton X
Fixative
Methanol:acetone
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: block buffer
Dilution
1/1000
Additional data
Additional Notes
Staining on WT cells shown (green is beta-catenin, blue is DAPI). I also stained a cell line known to have reduced beta-catenin expression and saw correspondingly reduced expression with this antibody (not shown).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 08 2014