Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Podocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Mouse Podocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBT + 4% Milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Licor Donkey anti-Rabbit 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Licor ECL
Negative control
No primary
Exposure
4 second(s)
Bands
Specific: 110 kDa Non-specific: 240 kDa
Positive control
Control Lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 27 2018