Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/250
Name
Abcam antibody:Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Frozen rat brain tissus were investigated from confocal microscopy
Abcam response
Thank you for your review using rat frozen tissue for IHC. We have not tested this rat, however testing from a collaborator worked well on mouse frozen tissue. In their protocol, unfixed cryosections were fixed in 4% Formalin in PBS before treatment with 70% Formic acid to most effectively reveal antigenic sites. This may improve your results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mehmet Sedat Feyat
Verified customer
投稿 Oct 02 2015