Anti-beta Actin 抗体 [mAbcam 8226] - Loading Control (HRP) (ab20272)

製品の概要

  • 製品名
    Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP)
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 8226] to beta Actin - Loading Control (HRP)
  • 由来種
    Mouse
  • 標識
    HRP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human, Pig, Drosophila melanogaster, African green monkey, Chinese hamster
    交差が予測される動物種: Sheep, Horse, Guinea pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.

  • ポジティブ・コントロール
    • HeLa whole cell lysate, 3T3 whole cell lysate, Rat brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab20272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.

ターゲット情報

画像

  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution

    Lane 1 : human testis lysate
    Lane 2 : human brain lysate
    Lane 3 : human tonsil lysate
    Lane 4 : mouse brain lysate
    Lane 5 : mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa


    Exposure time: 2 minutes
  • All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 3T3 whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds
  • Lanes 1-4 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution (Blocked in 5% BSA)
    Lanes 5-8 : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (HRP) (ab20272) at 1/5000 dilution (Blocked in 5% MILK)

    Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lanes 2 & 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lanes 3 & 7 : NIH 3T3 whole cell lysate (ab7179)
    Lanes 4 & 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 1 minute


    The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.

プロトコール

参考文献

This product has been referenced in:
  • Fiaturi N  et al. CCN5 in alveolar epithelial proliferation and differentiation during neonatal lung oxygen injury. J Cell Commun Signal 12:217-229 (2018). Read more (PubMed: 29349730) »
  • Welch RD  et al. Pharmacological inhibition of REV-ERB stimulates differentiation, inhibits turnover and reduces fibrosis in dystrophic muscle. Sci Rep 7:17142 (2017). WB . Read more (PubMed: 29215066) »

See all 101 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 29 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (15% PAGE)
Loading amount
50 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 29 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 29 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 20 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Spleen)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Spleen
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Sergi Bayod

Verified customer

投稿 Mar 24 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Cell lysate - whole cell (h9c2)
Specification
h9c2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (MEF)
Specification
MEF
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (hek293)
Specification
hek293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
40 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast epithelial)
Loading amount
25 µg
Specification
Breast epithelial
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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