Mouse, Rat, Human 交差が予測される動物種:
Cow, Drosophila melanogaster, Schizosaccharomyces pombe, Chinese hamster
Synthetic peptide corresponding to Human beta Actin aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)). (Peptide available as ab13772)
WB: HEK293, NIH3T3 and PC12 whole cell lysates. Human skeletal muscle tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more.
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Western blot - Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) (ab197277)
All lanes : Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) (ab197277) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa Observed band size : 42 kDa
Exposure time : 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.