Anti-beta Actin 抗体 [mAbcam 8224] - Loading Control (HRP) (ab197277)

製品の概要

  • 製品名
    Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP)
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 8224] to beta Actin - Loading Control (HRP)
  • 標識
    HRP
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Drosophila melanogaster, Schizosaccharomyces pombe, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human beta Actin aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) (Sulfosuccinimidyl 4-N-maleimidomethyl-cyclohexane-1-carboxylate (Sulfo-SMCC)).
    (Peptide available as ab13772)

  • ポジティブ・コントロール
    • WB: HEK293, NIH3T3 and PC12 whole cell lysates. Human skeletal muscle tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab197277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772).

This antibody has been designed for use as a loading control and is ideal for this purpose. Block membrane for 1 hr in 5% BSA. Incubate antibody in TBST for one hour or more.

ターゲット情報

  • 機能
    Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患
    Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 配列類似性
    Belongs to the actin family.
  • 翻訳後修飾
    ISGylated.
  • 細胞内局在
    Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin beta antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) 画像

  • All lanes : Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) (ab197277) at 1/5000 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 10 seconds

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197277 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

プロトコール

Anti-beta Actin antibody [mAbcam 8224] - Loading Control (HRP) (ab197277) 使用論文

ab197277 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab197277.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録