Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
14 µg
Specification
Lung
Blocking step
Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 23°C · Diluent: 50% Odyssey TBS Blocking Buffer+Tween
Dilution
1/5000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-infrared
Negative control
Primary antibody was omitted, which resulted in no bands (image not shown).
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 42 kDa
Positive control
Positive control was not included.
Additional data
Additional Notes
Note that this antibody was tested with 3 different blocking reagents, proprietary to Li-Cor: TBS Block, PBS Block, and Casein Block.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Pete Serbedzija
Verified customer
投稿 Apr 29 2016