Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
75000 cells
Specification
VERO E6
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/20000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 43 kDa
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投稿 Jun 28 2016