Application
Western blot
Sample
Rat Tissue lysate - whole (Fat-adipose tissue)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
20 µg
Specification
Fat-adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 3% milk (TBS-tween)
Dilution
1/20000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 42 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 09 2016