Anti-beta Actin 抗体 [8F10-G10] (ab170325)
Key features and details
- Mouse monoclonal [8F10-G10] to beta Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster
- Isotype: IgG2b
製品の概要
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製品名
Anti-beta Actin antibody [8F10-G10]
beta Actin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8F10-G10] to beta Actin -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Rabbit, Goat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish, African green monkey, Chinese hamster -
免疫原
Recombinant fragment corresponding to Human beta Actin. Expressed in E.coli.
Database link: P60709 -
ポジティブ・コントロール
- WB: COS7, K562, HeLa, 3T3 cell lysate.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Concentrated Culture Supernatant -
ポリ/モノ
モノクローナル -
クローン名
8F10-G10 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170325の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/5000. Predicted molecular weight: 42 kDa.
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特記事項 |
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WB
1/5000. Predicted molecular weight: 42 kDa. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
配列類似性
Belongs to the actin family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Entrez Gene: 100009272 Rabbit
- Entrez Gene: 81822 Rat
- Entrez Gene: 57934 Zebrafish
- Omim: 102630 Human
- SwissProt: P48975 Chinese hamster
- SwissProt: P60709 Human
see all -
別名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
画像
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : COS7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : C6 cell lysate
Lane 4 : 3T3 cell lysate
Predicted band size: 42 kDa -
All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/10000 dilution
Lane 1 : Goat muscle cell lysate
Lane 2 : CHO-K1 cell lysate
Lane 3 : COS7 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : 3T3 cell lysate
Lane 6 : K562 cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes :
Lane 1 : Rabbit spleen cell lysate
Lane 2 : Rabbit colon cell lysate
Lane 3 : Rabbit skeletal muscle cell lysate
Lane 4 : Rabbit liver cell lysate
Lane 5 : Rabbit heart cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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Lane 1 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 2 : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
All lanes : Zebrafish row cell lysate
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/1000 dilution
Lane 1 : Arabidopsis cell lysate at 30 µg
Lane 2 : Arabidopsis cell lysate at 15 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?This image was produced with antibody produced in ascites.
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All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/5000 dilution
Lane 1 : S. cerevisiae cell lysate at 10 µg
Lane 2 : P. pastoris cell lysate at 10 µg
Lane 3 : S. cerevisiae cell lysate at 5 µg
Lane 4 : P. pastoris cell lysate at 5 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes :
Lane 1 : COS7 cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : k562 cell lysate
Lane 4 : HeLa cell lysate
Predicted band size: 42 kDa -
All lanes : Anti-beta Actin antibody [8F10-G10] (ab170325) at 1/3000 dilution
Lane 1 : Drosophila melanogaster cell lysate at 40 µg
Lane 2 : Drosophila melanogaster cell lysate at 20 µg
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab170325 は 14 報の論文で使用されています。
- Lins BB et al. Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na+ Transporters Through the Nephron. Front Physiol 12:642752 (2021). PubMed: 33868007
- Zavala MR et al. Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development. Front Cardiovasc Med 8:622583 (2021). PubMed: 33718450
- Sahoo PK et al. Bisphenol A exposure induces neurobehavioral deficits and neurodegeneration through induction of oxidative stress and activated caspase-3 expression in zebrafish brain. J Biochem Mol Toxicol 35:e22873 (2021). PubMed: 34342104
- Campos OA et al. A pathogenic role for histone H3 copper reductase activity in a yeast model of Friedreich's ataxia. Sci Adv 7:eabj9889 (2021). PubMed: 34919435
- Meers C et al. Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ?. Mol Cell 79:1037-1050.e5 (2020). PubMed: 32882183