Anti-beta 3 Adrenergic Receptor 抗体 (ab94506)

製品の概要

  • 製品名Anti-beta 3 Adrenergic Receptor antibody
    beta 3 Adrenergic Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta 3 Adrenergic Receptor
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Mouse beta 3 Adrenergic Receptor aa 350 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab108460, ab108460)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in mouse brown adipose tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Pancreas.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 43 kDa).
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
  • 組織特異性Expressed mainly in adipose tissues.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADR B3 antibody
    • ADRB 3 antibody
    • ADRB3 antibody
    • ADRB3_HUMAN antibody
    • ADRB3R antibody
    • Adrenergic beta 3 receptor antibody
    • B3AR antibody
    • Beta 3 adrenoceptor antibody
    • Beta 3 adrenoreceptor antibody
    • Beta 3AR antibody
    • Beta-3 adrenergic receptor antibody
    • Beta-3 adrenoceptor antibody
    • Beta-3 adrenoreceptor antibody
    • Beta3AR antibody
    • FLJ99960 antibody
    see all

Anti-beta 3 Adrenergic Receptor antibody 画像

  • Anti-beta 3 Adrenergic Receptor antibody (ab94506) at 1 µg/ml + Brown Adipose (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab94506 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of beta 3 Adrenergic Receptor staining in Mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab94506, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-beta 3 Adrenergic Receptor antibody (ab94506) 使用論文

This product has been referenced in:
  • Shimizu I  et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
  • Wan Z  et al. IL-6 indirectly modulates the induction of glyceroneogenic enzymes in adipose tissue during exercise. PLoS One 7:e41719 (2012). Read more (PubMed: 22844518) »

See all 2 Publications for this product

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ab94506 is not tested in ICC/IF so it is eligible for testing discount. Are you planning using the knockout cells only or would like to try the normal cells also?

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Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (aortic endothelial cells)
Permeabilization Yes - Saponin 0,1%
Specification aortic endothelial cells
Blocking step PBS/Saponin0,1%/BSA5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative PFA 4%
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Miss. Virginie Joris

Verified customer

投稿 Jul 06 2015

DISCOUNT CODE: XXXXXXXX
Expiration date: 19-06-2012

I am very pleased to hear you would like to accept our offer and test ab94506 in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"