Anti-beta 3 Adrenergic Receptor 抗体 (ab59685)

製品の概要

  • 製品名Anti-beta 3 Adrenergic Receptor antibody
    beta 3 Adrenergic Receptor 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to beta 3 Adrenergic Receptor
  • アプリケーション適用あり: WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    LNRFDGYEGARPFPT

    , corresponding to amino acids 386-400 of Mouse beta 3 Adrenergic Receptor

  • ポジティブ・コントロール
    • Mouse brain extract MEF1 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 23283549ab37382-Chicken polyclonal IgY, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.
  • 組織特異性Expressed mainly in adipose tissues.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADR B3 antibody
    • ADRB 3 antibody
    • ADRB3 antibody
    • ADRB3_HUMAN antibody
    • ADRB3R antibody
    • Adrenergic beta 3 receptor antibody
    • B3AR antibody
    • Beta 3 adrenoceptor antibody
    • Beta 3 adrenoreceptor antibody
    • Beta 3AR antibody
    • Beta-3 adrenergic receptor antibody
    • Beta-3 adrenoceptor antibody
    • Beta-3 adrenoreceptor antibody
    • Beta3AR antibody
    • FLJ99960 antibody
    see all

Anti-beta 3 Adrenergic Receptor antibody 画像

  • ICC/IF image of ab59685 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59685, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96947, DyLight® 488 goat anti-chicken IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-beta 3 Adrenergic Receptor antibody (ab59685) 使用論文

This product has been referenced in:
  • Okumura T  et al. Phospholipase C-Related Catalytically Inactive Protein (PRIP) Regulates Lipolysis in Adipose Tissue by Modulating the Phosphorylation of Hormone-Sensitive Lipase. PLoS One 9:e100559 (2014). WB ; Mouse . Read more (PubMed: 24945349) »
  • Salisbury EA  et al. Transient brown adipocyte-like cells derive from peripheral nerve progenitors in response to bone morphogenetic protein 2. Stem Cells Transl Med 1:874-85 (2012). Flow Cyt ; Mouse . Read more (PubMed: 23283549) »

See all 2 Publications for this product

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The antibodies ab101095 and ab59685 are fully characterized with rat sample and both are guaranteed for western blot. The differences between the two is in concentration, host species, storage buffer and immunogen ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (white adipose tissue)
Loading amount 50 µg
Specification white adipose tissue
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"