Anti-beta 2 Microglobulin 抗体 [B2M-02] (ab27588)

製品の概要

  • 製品名Anti-beta 2 Microglobulin antibody [B2M-02]
    beta 2 Microglobulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B2M-02] to beta 2 Microglobulin
  • 特異性Ab27588 reacts with beta 2 Microglobulin associated with cell surface MHC Class I molecules and other membrane antigens as well as with soluble beta2 microglobulin.
  • アプリケーション適用あり: Flow Cyt, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Human, Pig
  • 免疫原

    Purified isolated human beta 2 Microglobulin.

  • 特記事項

    The antibody is free of unconjugated biotin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 15mM Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Ab27588 was purified from ascites by affinity chromatography. > 95% (by SDS PAGE)
  • ポリ/モノモノクローナル
  • クローン名B2M-02
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.

ターゲット情報

  • 機能Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
  • 関連疾患Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
  • 配列類似性Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 翻訳後修飾Glycation of Ile-21 is observed in long-term hemodialysis patients.
  • 細胞内局在Secreted. Detected in serum and urine.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B2M antibody
    • B2MG_HUMAN antibody
    • Beta 2 microglobin antibody
    • Beta 2 microglobulin antibody
    • Beta 2 microglobulin precursor antibody
    • Beta chain of mhc class 1 proteins antibody
    • Beta chain of MHC class I molecules antibody
    • Beta-2-microglobulin form pI 5.3 antibody
    • CDABP0092 antibody
    • Hdcma22p antibody
    see all

Anti-beta 2 Microglobulin antibody [B2M-02] 画像

  • Flow cytometry analysis of human peripheral blood cells labelling beta2-microglobulin with ab27588.

  • Ab27588 staining human normal skin tissue. Staining is localised to cell membrane.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required

Anti-beta 2 Microglobulin antibody [B2M-02] (ab27588) 使用論文

This product has been referenced in:
  • Drbal K  et al. A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood 98:1561-6 (2001). Read more (PubMed: 11520808) »
  • Hilgert I  et al. The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin. Folia Biol (Praha) 30:369-76 (1984). Read more (PubMed: 6394369) »

See all 2 Publications for this product

Product Wall

Thank you for your reply.

Based on the information that you have provided, there are a few suggestions I have that may resolve this issue:

1 - From the information provided by Uniprot for human (http://www.uniprot.org/uniprot/P61769...

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Thank you for contacting us.

I am sorry that this product is not performing in a satisfactory manner for you and would like to thank you for providing details and images. If you could please provide me with protocol details and order infor...

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Thank you for contacting us. Unfortunately we currently do not have any antibodies against porcine MHC class I. However we do have an antibody which will react with Porcine β2-microglobulin: Anti-beta 2 Microglobulin (ab27588) www.abcam...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"