製品の概要

  • 製品名Anti-beta 2 Defensin antibody
    beta 2 Defensin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to beta 2 Defensin
  • アプリケーション適用あり: WB, Sandwich ELISA, Neutralisingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human beta 2 Defensin aa 24-64. Highly pure (>98%) recombinant Human beta 2 Defensin. Full length mature Beta-defensin 2 used as immunogen.
    Sequence:

    GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P


    Database link: O15263

  • ポジティブ・コントロール
    • recombinant hBD-2 (human Beta Defensin-2) ab9872
  • 特記事項

    hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.

製品の特性

  • 製品の状態Lyophilised:Centrifuge vial prior to opening. Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described, then no further reconstitution is necessary. The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 7.2
    Constituent: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.

To detect beta 2 Defensin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant beta 2 Defensin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

To detect beta 2 Defensin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab83509 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant beta 2 Defensin.

Neutralising Use a concentration of 5 - 8 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of Human Beta 2 Defensin (1.0 μg/ml), a concentration of 5.0 - 8.0 μg/ml of this antibody is required.

ターゲット情報

  • 機能Has antibacterial activity.
  • 組織特異性Expressed in the skin and respiratory tract.
  • 配列類似性Belongs to the beta-defensin family. LAP/TAP subfamily.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BD 2 antibody
    • BD-2 antibody
    • BD2 antibody
    • beta 2 antibody
    • Beta defensin 2 antibody
    • Beta defensin 4B antibody
    • Beta-defensin 2 antibody
    • Beta-defensin 4A antibody
    • DEF B2 antibody
    • DEF B4 antibody
    • DEFB 102 antibody
    • DEFB 2 antibody
    • DEFB 4 antibody
    • DEFB102 antibody
    • DEFB2 antibody
    • DEFB2, formerly antibody
    • DEFB4 antibody
    • DEFB4, formerly antibody
    • DEFB4B antibody
    • DEFB4P antibody
    • Defensin antibody
    • Defensin beta 2 antibody
    • Defensin, beta 4 antibody
    • Defensin, beta 4, pseudogene antibody
    • Defensin, beta 4A antibody
    • Defensin, beta 4B antibody
    • Defensin, beta, 2, formerly antibody
    • Defensin, beta, 4, formerly antibody
    • DFB4A_HUMAN antibody
    • HBD 2 antibody
    • hBD-2 antibody
    • HBD2 antibody
    • mBD-2 antibody
    • SAP 1 antibody
    • SAP1 antibody
    • Skin antimicrobial peptide 1 antibody
    • Skin-antimicrobial peptide 1 antibody
    see all

Anti-beta 2 Defensin antibody (ab9871) 使用論文

This product has been referenced in:
  • de Koning HD  et al. A comprehensive analysis of pattern recognition receptors in normal and inflamed human epidermis: upregulation of dectin-1 in psoriasis. J Invest Dermatol 130:2611-20 (2010). Read more (PubMed: 20631729) »
  • Pignatti P  et al. Tracheostomy and related host-pathogen interaction are associated with airway inflammation as characterized by tracheal aspirate analysis. Respir Med 103:201-8 (2009). Read more (PubMed: 18980836) »

See all 4 Publications for this product

Product Wall

The exact immunogen sequence for these antibodies is proprietary so I am sorry we are unable to disclose this information. The immunogen however corresponds to the mature form of Beta 2 defensin protein. We do have sequence listed for ab14426 and ab66...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (supernatens from keratinocytes)
Specification supernatens from keratinocytes
Type Direct
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Thank you for your interest in Abcam.

We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services...

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Thank you for submitting the Abreviews with ab9871, ab15117, ab110534 and ab18499 using dot blot. The results look really good.

I will be submitting your reviews manually. In order to issue the Abpoints generated to the relevant account, cou...

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Thank you for contacting us.

As discussed we have many anti goat antibodies that can be used in ELISA and ICC/IF.

Idealy enzyme conjugated antibodies e.g. HRP, Alkaline phosphatase are the best suitable antibodies in ELISA. However...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (keratinocytes)
Loading amount 500000 cells
Specification keratinocytes
Treatment see below (Additional notes)
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 27 2012

DISCOUNT CODE: xxxxx Expiration date: 31/03/2012 I am very pleased to hear your customer would like to test ab9871 in Dot Blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abr...

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Thank you for contacting us. We would be very interested in your customer testing ab9871 with Dot Blot with human samples. If you could please send me the name, email address and institute of your customer I will create the discount code for you.

Thank you for your response and your interest. We look forward to receiving your feedback on ab9871 and publishing the Abreview. We are unable to issue discount codes for testing in retrospect for those antibodies which were purchased in the past. ...

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This antibody is produced using Recombinant Human beta-defensin-2 (BD-2) as the immunizing antigen. BD-2 is an antimicrobial peptide which belongs to the distinct family of beta-defensins. Recent studies have shown that the beta-defensins are present i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"