Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (12% Tris-HCl)
Sample
Human Cell lysate - whole cell (MDCK cell lysate transfected with human, mouse and)
Specification
MDCK cell lysate transfected with human, mouse and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-T, 5% Milk
Dilution
1/10000
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: ~10 kDa Non-specific: none kDa
Exposure
3 minute(s) and 0 second(s)
Negative control
Untransfected MDCK cells
Positive control
Human cell lysate
Additional data
Additional Notes
PVDF membrane, cell lysis buffer was PBS, 0.5% CHAPS, + Complete EDTA-free protease inhibitor tablet
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jonathan
Verified customer
投稿 Jul 30 2013