Biotin Anti-beta 2 Microglobulin 抗体 [B2M-01] (ab21899)
Key features and details
- Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-beta 2 Microglobulin antibody [B2M-01]
beta 2 Microglobulin 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin -
由来種
Mouse -
標識
Biotin -
特異性
This antibody reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin. -
アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human
非交差種: Mouse, Rabbit, Chicken, Cow, Dog -
免疫原
Full length native protein (purified) (Human).
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ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung. Flow Cytometry: Human peripheral blood.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
B2M-01 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 10 µg/ml. |
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
ターゲット情報
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機能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
関連疾患
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
配列類似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻訳後修飾
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
細胞内局在
Secreted. Detected in serum and urine. - Information by UniProt
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参照データベース
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
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別名
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
画像
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Flow cytometry analysis using anti-beta2 microglobulin (B2M-01) biotin for surface staining of beta2-microglobulin in human peripheral blood
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IHC image of beta 2 Microglobulin staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21899, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab21899 は 2 報の論文で使用されています。
- Zhao H et al. A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods 59:328-35 (2013). PubMed: 23270815
- Andersen JT et al. FcRn binding properties of an abnormal truncated analbuminemic albumin variant. Clin Biochem 43:367-72 (2010). ELISA ; Human . PubMed: 20006594