Application
Western blot
Sample
Human Cell lysate - whole cell (keratinocytes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Loading amount
500000 cells
Treatment
see below (Additional notes)
Specification
keratinocytes
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer (5%BSA in TBST)
Concentration
1 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Donkey anti-goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescence
Exposure
45 minute(s) and 0 second(s)
Bands
Specific: 7 kDa
Positive control
synthetic HBD-2 (50nmol)
Additional data
Additional Notes
1 = positive control (synthetic HBD-2 (50nmol))
2 = keratinocytes from healthy subjects - untreated
3 = keratinocytes from healthy subjects - traeted with C. albicans
4 = keratinocytes from healthy subjects - traeted with Staph. aureus
5 = keratinocytes from AD (atopic dermatitis) patient
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 27 2012