Anti-beta 1 Sodium Potassium ATPase 抗体 [464.8] (ab8344)

製品の概要

  • 製品名
    Anti-beta 1 Sodium Potassium ATPase antibody [464.8]
    beta 1 Sodium Potassium ATPase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [464.8] to beta 1 Sodium Potassium ATPase
  • アプリケーション
    適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Rat, Cow, Dog, Human, Pig
  • 免疫原

    Purified Na,K-ATPase from rabbit renal outermedulla

  • ポジティブ・コントロール
    • ab8344 gave a positive signal in human, mouse and rat kidney tissue lysates. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能
    This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
  • 組織特異性
    Found in most tissues.
  • 配列類似性
    Belongs to the X(+)/potassium ATPases subunit beta family.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adenosinetriphosphatase antibody
    • AT1B1_HUMAN antibody
    • ATP 1B antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • Atpb 1 antibody
    • ATPBS antibody
    • Beta 1 subunit of Na(+) K(+) ATPase antibody
    • Na K ATPase beta 1 polypeptide antibody
    • Na+/K+ ATPase beta 1 subunit antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • Sodium/potassium-dependent ATPase subunit beta-1 antibody
    • Sodium/potassium-transporting ATPase subunit beta-1 antibody
    see all

画像

  • Anti-beta 1 Sodium Potassium ATPase antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg

    Secondary
    Granzyme B overexpression lysate (ab94040) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Wen X  et al. Gene-expression profile and localization of Na+/K(+)-ATPase in rat enamel organ cells. Eur J Oral Sci 122:21-6 (2014). Read more (PubMed: 24313748) »
  • Lu Q  et al. Ca²?/calmodulin-dependent protein kinase II contributes to hypoxic ischemic cell death in neonatal hippocampal slice cultures. PLoS One 8:e70750 (2013). WB ; Rat . Read more (PubMed: 23976956) »

See all 12 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (enamel organ)
Loading amount
15 µg
Specification
enamel organ
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 18 2013

I can confirm that the laboratory has stopped using the Oddyssey blocking buffer as it gave a number of signal problems. We recommend to use 5% BSA or 5% milk, and both should work with the Oddyssey machine according to the lab. Can you please clari...

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Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from...

Read More

This antibody has been shown to cross react with Cow, Dog, Human, Pig and Rat. I'm afraid that, to our knowledge, it has not yet been tested in other species, so I do not know if it will work with CHO cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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