製品の概要
-
製品名Anti-beta 1 Adrenergic Receptor antibody
beta 1 Adrenergic Receptor 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to beta 1 Adrenergic Receptor
-
由来種Rabbit
-
アプリケーション適用あり: ICC/IF, WBmore details
-
種交差性交差種: Mouse
交差が予測される動物種: Sheep, Cow, Cat, Human, Pig
-
免疫原
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse beta 1 Adrenergic Receptor.
(Peptide available as ab97424.)
-
ポジティブ･コントロール
- This antibody gave a positive signal in Mouse Spinal cord tissue lysate.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
-
ポリ/モノポリクローナル
-
アイソタイプIgG
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab85037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
-
機能Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
-
配列類似性Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily.
-
ドメインThe PDZ domain-binding motif mediates competitive interactions with GOPC, MAGI3 and DLG4 and plays a role in subcellular location of the receptor.
-
翻訳後修飾Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.
-
細胞内局在Cell membrane. Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression.
- Information by UniProt
-
参照データベース
- Entrez Gene: 493971 Cat
- Entrez Gene: 281604 Cow
- Entrez Gene: 153 Human
- Entrez Gene: 11554 Mouse
- Entrez Gene: 397355 Pig
- Omim: 109630 Human
- SwissProt: Q9TST6 Cat
- SwissProt: Q9TT96 Cow
see all -
別名
- ADR B1 antibody
- ADRB 1 antibody
- ADRB1 antibody
see all
画像
-
Western blot - beta 1 Adrenergic Receptor antibody (ab85037)Anti-beta 1 Adrenergic Receptor antibody (ab85037) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
Exposure time: 20 minutes
Mouse Beta-1 adrenergic receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - beta 1 Adrenergic Receptor antibody (ab85037)ICC/IF image of ab85037 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85037, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
参考文献
This product has been referenced in:
- Shimizu I et al. Vascular rarefaction mediates whitening of brown fat in obesity. J Clin Invest 124:2099-112 (2014). WB ; Mouse . Read more (PubMed: 24713652) »
- Sugatani J et al. Antiobese function of platelet-activating factor: increased adiposity in platelet-activating factor receptor-deficient mice with age. FASEB J 28:440-52 (2014). Read more (PubMed: 24100020) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"