製品の概要

  • 製品名Anti-Bestrophin antibody
    Bestrophin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bestrophin
  • アプリケーション適用あり: IP, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human, Cynomolgus Monkey
  • 免疫原

    Synthetic peptide, corresponding to C term. of Human Bestrophin

  • ポジティブ・コントロール
    • Conditioned medium cultured RPE cells (the basal expression of Bestrophin is low in cultured cells).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/200.
WB 1/500. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.
  • 組織特異性Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
  • 関連疾患Defects in BEST1 are the cause of vitelliform macular dystrophy type 2 (VMD2) [MIM:153700]; also known as Best macular dystrophy (BMD). VMD2 is an autosomal dominant form of macular degeneration that usually begins in childhood or adolescence. VMD2 is characterized by typical 'egg-yolk' macular lesions due to abnormal accumulation of lipofuscin within and beneath the retinal pigment epithelium cells. Progression of the disease leads to destruction of the retinal pigment epithelium and vision loss.
    Defects in BEST1 are the cause of retinitis pigmentosa type 50 (RP50) [MIM:613194]. A retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in BEST1 are a cause of adult-onset vitelliform macular dystrophy (AVMD) [MIM:608161]. AVMD is a rare autosomal dominant disorder with incomplete penetrance and highly variable expression. Patients usually become symptomatic in the fourth or fifth decade of life with a protracted disease of decreased visual acuity.
    Defects in BEST1 are the cause of bestrophinopathy autosomal recessive (ARB) [MIM:611809]. A retinopathy characterized by central visual loss, an absent electro-oculogram light rise, and a reduced electroretinogram.
    Defects in BEST1 are the cause of vitreoretinochoroidopathy autosomal dominant (ADVIRC) [MIM:193220]. A disorder characterized by vitreoretinochoroidal dystrophy. The clinical presentation is variable and may be associated with cataract, nanophthalmos, microcornea, shallow anterior chamber, and glaucoma.
  • 配列類似性Belongs to the bestrophin family.
  • 翻訳後修飾Phosphorylated by PP2A.
  • 細胞内局在Cell membrane. Basolateral cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARB antibody
    • BEST 1 antibody
    • BEST antibody
    • Best disease antibody
    • Best macular dystrophy antibody
    • BEST1 antibody
    • BEST1_HUMAN antibody
    • Best1V1Delta2 antibody
    • Bestrophin 1 antibody
    • Bestrophin-1 antibody
    • Bestrophin1 antibody
    • BMD antibody
    • mBest1 antibody
    • RP50 antibody
    • TU15B antibody
    • Vitelliform macular dystrophy 2 antibody
    • Vitelliform macular dystrophy antibody
    • Vitelliform macular dystrophy protein 2 antibody
    • VMD 2 antibody
    • VMD2 antibody
    see all

Anti-Bestrophin antibody 画像

  • IHC image of ab14929 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14929, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Bestrophin antibody (ab14929) 使用論文

This product has been referenced in:
  • Sohn EH  et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep 5:11791 (2015). IHC-Fr . Read more (PubMed: 26138532) »
  • Tucker BA  et al. Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa. Elife 2:e00824 (2013). IHC . Read more (PubMed: 23991284) »

See all 4 Publications for this product

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I have checked the sequence similarity between human and rat proteins based on this similarity, my recommendations about the rat cross reactivity would be

- The sequence is 94% similar between ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"