製品の概要

  • 製品名Anti-Bestrophin 3 antibody
  • 製品の詳細
    Rabbit polyclonal to Bestrophin 3
  • 特異性The immunogen shows 100% homology with Bestrophin 3 isoforms 1 and 2. The immunogen also shows 76% homology with bestrophin 1 and 68% bestrophin 2. We predict it will react based on similar homology, but this cross-reactivity has not been tested.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (LVYTQVAEQL INPFGEDDDD FETNWCIDRN LQVSLLAVDE MHMSLPKMKK) of Human Bestrophin 3 (NP_689652).

  • ポジティブ・コントロール
    • Human fetal muscle lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab94904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Forms calcium-sensitive (By similarity) chloride channels. Permeable to bicarbonate.
  • 組織特異性Present in skeletal muscle and weakly in brain, spinal cord, bone marrow and retina.
  • 配列類似性Belongs to the bestrophin family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BEST 3 antibody
    • BEST3 antibody
    • BEST3_HUMAN antibody
    • Bestrophin-3 antibody
    • Bestrophin3 antibody
    • MGC13168 antibody
    • MGC40411 antibody
    • OTTHUMP00000195114 antibody
    • OTTHUMP00000233491 antibody
    • Vitelliform macular dystrophy 2 like 3 antibody
    • Vitelliform macular dystrophy 2 like protein 3 antibody
    • Vitelliform macular dystrophy 2-like protein 3 antibody
    • VMD2L3 antibody
    see all

Anti-Bestrophin 3 antibody 画像

  • Anti-Bestrophin 3 antibody (ab94904) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
  • Anti-Bestrophin 3 antibody (ab94904) at 1/500 dilution (in PBS-T (0.1%) for 18 hours at 4°C) + Mouse heart whole tissue lysate at 30 µg

    Secondary
    A Biotin-conjugated Goat anti-rabbit IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 76.4 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Bestrophin 3 antibody (ab94904) 使用論文

ab94904 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (heart)
Loading amount 30 µg
Specification heart
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
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投稿 Sep 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"