The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
BEST4 (Bestrophin 4) is a member of the bestrophin gene family of anion channels. Bestrophins are transmembrane spanning proteins that share a homology region with a high content of aromatic residues including an invariant arginine (R), phenylalanine (F), and proline (P) motif. BEST4 may form calcium-sensitive chloride channels and may conduct other physiologically significant anions such as bicarbonate.