製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Beclin 1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 1-50 of Human Beclin 1 (NP_003757.1)

  • ポジティブ・コントロール
    • HeLa, 293T or NIH3T3 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
    • 組織特異性
      Ubiquitous.
    • 配列類似性
      Belongs to the beclin family.
    • 細胞内局在
      Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • APG6 antibody
      • ATG 6 antibody
      • ATG6 antibody
      • ATG6 autophagy related 6 homolog antibody
      • Bcl-2-interacting protein beclin antibody
      • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
      • Beclin 1 autophagy related antibody
      • Beclin-1 antibody
      • Beclin1 antibody
      • BECN 1 antibody
      • Becn1 antibody
      • BECN1_HUMAN antibody
      • Coiled coil myosin like BCL2 interacting protein antibody
      • Coiled-coil myosin-like BCL2-interacting protein antibody
      • GT197 antibody
      • Protein GT197 antibody
      • VPS 30 antibody
      • VPS30 antibody
      see all

    画像

    • All lanes : Anti-Beclin 1 antibody (ab92389)

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 52 kDa


      Exposure time : 3 minutes
    • Sample: HeLa whole cell lysate (1mg for IP, 20% of IP loaded).
      Antibodies: ab92389 used for WB 1µg/ml (lane 1) and used for IP at 3µg/mg lysate. Lane 2: control IgG.
      Detection: chemiluminescence with exposure time of 10 seconds.

    参考文献

    This product has been referenced in:

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab92389.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録