製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Beclin 1
  • アプリケーション
    適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    17 amino acid synthetic peptide from near the amino terminus of human Beclin 1

  • ポジティブ・コントロール
    • WB: HEK-293 cell lysate. IHC-P: Human and rat brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the beclin family.
  • 細胞内局在
    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody 画像

  • Paraffin-embedded human brain tissue stained for Beclin 1 using ab62557 at 2.5 µg/ml in immunohistochemical analysis.

  • Lane 1 : Anti-Beclin 1 antibody (ab62557) at 0.5 µg/ml
    Lane 2 : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
    Lane 3 : Anti-Beclin 1 antibody (ab62557) at 2 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab62557 staining Beclin 1 in Human MCR5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 21°C. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab62557 staining Beclin 1 in Human umbilical vascular endothelial cells after 2 Gy of ionizing radiationby at 4 and 8 days post-irradiation by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 3.7% formaldehyde/PBS pH 7.4 for 20 minutes at 37°C, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% PBS with BSA pH 7.4. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. An undiluted Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal was used as the secondary antibody.

  • Paraffin-embedded rat brain tissue stained for Beclin 1 using ab62557 at 2 µg/ml in immunohistochemical analysis.

Anti-Beclin 1 antibody (ab62557) 使用論文

This product has been referenced in:
  • Huang G  et al. Notoginsenoside R1 attenuates glucose-induced podocyte injury via the inhibition of apoptosis and the activation of autophagy through the PI3K/Akt/mTOR signaling pathway. Int J Mol Med 39:559-568 (2017). Human . Read more (PubMed: 28112381) »
  • Thapalia BA  et al. Sauchinone augments cardiomyocyte viability by enhancing autophagy proteins -PI3K, ERK(1/2), AMPK and Beclin-1 during early ischemia-reperfusion injury in vitro. Am J Transl Res 8:3251-65 (2016). WB ; Rat . Read more (PubMed: 27508047) »

See all 11 Publications for this product

Product Wall

Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Treatment
Perifosine
Specification
Epithelial OVCA cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jul 18 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (H1299)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Treatment
different concentrations of a drug
Specification
H1299
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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投稿 Jan 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (GONAD)
Antigen retrieval step
Enzymatic
Permeabilization
No
Specification
GONAD
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Jun 22 2015

Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Cell lysate - whole cell (gonad)
Specification
gonad
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jan 19 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (MRC5)
Specification
MRC5
Permeabilization
Yes - /0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative
Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

投稿 Nov 13 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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