Anti-Beclin 1 抗体 [OTI4A10] (ab118148)
Key features and details
- Mouse monoclonal [OTI4A10] to Beclin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Beclin 1 antibody [OTI4A10]
Beclin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI4A10] to Beclin 1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human Beclin 1. Produced in HEK-293T cells. NP_003757
Database link: Q14457 -
ポジティブ・コントロール
- WB: Beclin 1 transfected HEK-293T cell lysate; HeLa cell lysate.
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特記事項
The clone number has been updated from 4A10 to OTI4A10, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI4A10 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118148の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 52 kDa.
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特記事項 |
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WB
1/2000. Predicted molecular weight: 52 kDa. |
ターゲット情報
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機能
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the beclin family. -
細胞内局在
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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参照データベース
- Entrez Gene: 8678 Human
- Omim: 604378 Human
- SwissProt: Q14457 Human
- Unigene: 716464 Human
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別名
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
画像
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All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/2000 dilution
Lane 1 : Wild-type Jurkat cell lysate
Lane 2 : BECN1 CRISPR-Cas9 edited Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDaFalse colour image of Western blot: Anti-Beclin 1 antibody [OTI4A10] staining at 1/2000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab118148 was shown to bind specifically to Beclin 1. A band was observed at 52 kDa in wild-type Jurkat cell lysates with no signal observed at this size in BECN1 CRISPR-Cas9 edited cell line ab283778 (BECN1 CRISPR-Cas9 edited cell lysate ab283792). The band observed in the CRISPR-Cas9 edited lysate lane above 52 kDa is likely to represent Beclin 1 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and BECN1 CRISPR-Cas9 edited Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : BECN1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab118148 observed at 60 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab118148 was shown to react with Beclin 1 in wild-type HeLa cells in western blot. Loss of signal was observed when BECN1 knockout sample was used. Wild-type HeLa and BECN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab118148 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY Beclin 1
Lysates/proteins at 5 µg per lane.
Predicted band size: 52 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab118148 は 1 報の論文で使用されています。
- Blaszczak W et al. Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro. Molecules 23:N/A (2018). PubMed: 30545161