製品の概要

  • 製品名
    Anti-Beclin 1 antibody [2A4]
    Beclin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A4] to Beclin 1
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified recombinant fragment of human Beclin 1 expressed in E. Coli.

  • ポジティブ・コントロール
    • WB: Hela; A431; MCF7; RAJI; Jurkat and SKBR3 cell lysates IHC-P: Breast cancer and liver cancer tissues

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 60 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the beclin family.
  • 細胞内局在
    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APG6 antibody
    • ATG 6 antibody
    • ATG6 antibody
    • ATG6 autophagy related 6 homolog antibody
    • Bcl-2-interacting protein beclin antibody
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein) antibody
    • Beclin 1 autophagy related antibody
    • Beclin-1 antibody
    • Beclin1 antibody
    • BECN 1 antibody
    • Becn1 antibody
    • BECN1_HUMAN antibody
    • Coiled coil myosin like BCL2 interacting protein antibody
    • Coiled-coil myosin-like BCL2-interacting protein antibody
    • GT197 antibody
    • Protein GT197 antibody
    • VPS 30 antibody
    • VPS30 antibody
    see all

Anti-Beclin 1 antibody [2A4] 画像

  • All lanes : Anti-Beclin 1 antibody [2A4] (ab114071) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : Jurkat cell lysate
    Lane 6 : SKBR3 cell lysate


    Predicted band size : 60 kDa
  • Immunohistochemical analysis of Beclin 1 expression in paraffin embedded breast cancer tissues (left) and liver cancer tissues (right) using ab114071 at 1/200 dilution with DAB staining
  • Flow cytometric analysis of RAJI cells using Beclin 1 mouse mAb (green) and negative control (purple).

Anti-Beclin 1 antibody [2A4] (ab114071) 使用論文

This product has been referenced in:
  • Liu H  et al. PI3K/AKT/mTOR pathway promotes progestin resistance in endometrial cancer cells by inhibition of autophagy. Onco Targets Ther 10:2865-2871 (2017). WB ; Human . Read more (PubMed: 28652768) »
  • Di Fazio P  et al. Autophagy-related cell death by pan-histone deacetylase inhibition in liver cancer. Oncotarget 7:28998-9010 (2016). WB, IF, IHC . Read more (PubMed: 27058414) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (Canine osteosarcoma & mammary tumour)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
15 µg
Treatment
1-2 (OS1) = N/A, 3 (D17) = 75 uM HCQ, 4 (D17) = 75 uM HCQ + doxorubicin, 5 (D17) = 75 uM HCQ + 5 uM Spautin-1, 6 (D17) = 75 uM HCQ + 5 uM Spautin-1 + doxorubicin, 7 (CF41) = N/A, 8 (MCF7) = N/A, 9 (D17) = N/A, 10 (D17) = doxorubicin, 11 (D17) = 15 uM Spautin-1 + doxorubicin, 12 (D17) = 120 uM Spautin-1 + doxorubicin, 13 (SKBR3) = N/A, 14 (OS2) = E64d + pepstatin-A (All treatments 6 hr except lane 14 which was 24 hr)
Specification
Canine osteosarcoma & mammary tumour
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Courtney Schott

Verified customer

投稿 Sep 26 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% troton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 02 2016

Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12)
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Md. Golam Sharoar

Verified customer

投稿 Feb 16 2016

Thank you for contacting us.

Your credit note ID is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask...

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Thank you for contacting us.

My colleague John has forwarded you the Flow Cytometry protocol used by our lab, please let us know if you have any further questions about this.

Unfortunately, the concentration for this antibody was no...

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Thank you for contacting Abcam yesterday.

Please find attached the protocol for Flow Cytometry that was used to test ab114071. The secondary antibody used was a AlexaFluor@488goatanti-mouse(IgG(H+C), dilution:1:150.
Please let me know if...

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Code de remise : ********
Date d’expiration : JJ MM AAAA
Je suis ravi que vous souhaitiez participer à notre offre et tester ab114071 en ICC/IF. Le code de réduction correspondant est ******. Ce code de réduction est utilisable sous 120 jours...

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I apologize, I had sent the wrong link.  Here are the two references for ab114071: http://www.abcam.com/Beclin-1-antibody-2A4-ab114071-references.html

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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