製品の概要

  • 製品名Anti-BDP / ARID3B antibody
    BDP / ARID3B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BDP / ARID3B
  • 特異性Block in 3% milk
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse BDP/ ARID3B.

    (Peptide available as ab30607.)

  • ポジティブ・コントロール
    • F9 Mouse Embryonic Carcinoma, E14tG2a (Mouse embryonic stem cell line), PC12 (Rat adrenal pheochromocytoma cell line)Whole Cell Lysate; Mouse Thymus, Testis, Thyroid Gland and Cerebellum Tissue Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 61 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Transcription factor which may be involved in neuroblastoma growth and malignant transformation. Favors nuclear targeting of ARID3A.
  • 組織特異性Expressed in placenta, testis and leukocytes. Expressed in neuroblastoma. Present in K562 erythrocytic leukemia cells (at protein level).
  • 配列類似性Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARI3B_HUMAN antibody
    • ARID domain containing protein 3B antibody
    • ARID domain-containing protein 3B antibody
    • Arid3b antibody
    • AT rich interactive domain 3B BRIGHT like antibody
    • AT rich interactive domain containing protein 3B antibody
    • AT-rich interactive domain-containing protein 3B antibody
    • Bright and dead ringer gene product homologous protein Bdp antibody
    • Bright and dead ringer protein antibody
    • Bright like antibody
    • Bright-like protein antibody
    • Brightlike antibody
    • DRIL2 antibody
    see all

Anti-BDP / ARID3B antibody 画像

  • ICC/IF image of ab32481 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32481, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-BDP / ARID3B antibody (ab32481) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : Thyroid Gland (Mouse) Tissue Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Thymus (Mouse) Tissue Lysate
    Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : Cerebellum (Mouse) Tissue Lysate
    Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61,70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
  • IHC image of ab32481 staining in mouse testes formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32481, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-BDP / ARID3B antibody (ab32481) 使用論文

This product has been referenced in:

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