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RabMAb

Anti-BDNF 抗体 [EPR1292] (ab108319)

製品の概要

  • 製品名
    Anti-BDNF antibody [EPR1292]
    BDNF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1292] to BDNF
  • 由来種
    Rabbit
  • 特異性
    This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human BDNF aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P23560
    (Peptide available as ab182199)

  • ポジティブ・コントロール
    • WB: Human, rat and mouse hippocampus lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells
  • 特記事項

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/30.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with Human BDNF peptide (ab182199).
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

For unpurified use at 1/100 - 1/250 dilution. 

ICC/IF 1/500.

For unpurified use at 1/750 dilution. 

ターゲット情報

  • 機能
    During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
  • 組織特異性
    Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
  • 関連疾患
    Bulimia nervosa 2
    Congenital central hypoventilation syndrome
  • 配列類似性
    Belongs to the NGF-beta family.
  • 翻訳後修飾
    The propeptide is N-glycosylated and glycosulfated.
    Converted into mature BDNF by plasmin (PLG).
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abrineurin antibody
    • ANON2 antibody
    • BDNF antibody
    • BDNF_HUMAN antibody
    • Brain Derived Neurotrophic Factor antibody
    • Brain-derived neurotrophic factor antibody
    • BULN2 antibody
    • MGC34632 antibody
    • Neurotrophin antibody
    see all

画像

  • All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution (unpurified)

    Lane 1 : Human hippocampus lysate
    Lane 2 : Rat hippocampus lysate
    Lane 3 : Mouse hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Gt anti Rb IR680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Additional bands at: 15 kDa (possible mature (processed) protein), 28 kDa (possible multimer), 35 kDa, 45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with unpurified ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with Purified ab108319 at 1:500 (0.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BDNF with purified ab108319 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 80% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BDNF with Purified ab108319 at 1:500 dilution (0.56 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using unpurified ab108319 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with unpurified ab108319 at a dilution of 1/750. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

    The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

    See Abreview

参考文献

This product has been referenced in:
  • Fu F  et al. NDUFA4 enhances neuron growth by triggering growth factors and inhibiting neuron apoptosis through Bcl-2 and cytochrome C mediated signaling pathway. Am J Transl Res 10:164-174 (2018). Read more (PubMed: 29423002) »
  • Sutton LP  et al. Orphan receptor GPR158 controls stress-induced depression. Elife 7:N/A (2018). Read more (PubMed: 29419376) »

See all 39 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Endometrium (eutopic and ectopic))
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Endometrium (eutopic and ectopic)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 May 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1
Permeabilization
No
Specification
cerebellum
Fixative
formalin
Username

Dr. Emmanuel Bouchaert

Verified customer

投稿 Oct 06 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Sep 29 2016

Thank you for contacting us.
We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
I hope this information is helpful to you. Please d...

Read More

Thank you for contacting us and for your interest in our products. I think ab108319 (www.abcam.com/ab108319) or ab108383 (www.abcam.com/ab108383) would be suitable since you are looking for a monoclonal antibody. However, please note that none of t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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