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RabMAb

Anti-BDNF 抗体 [EPR1292] (ab108319)

製品の概要

  • 製品名
    Anti-BDNF antibody [EPR1292]
    BDNF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1292] to BDNF
  • 特異性
    This product may cross react with the following family members: NGF beta, neurotrophin 3, neurotrophin 4.
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human BDNF aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P23560
    (Peptide available as ab182199)

  • ポジティブ・コントロール
    • WB: U87-MG, C6, SH-SY5Y, Neuron-2a, PC-12 cell lysate, brain tissue lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells
  • 特記事項

    Our internal data indicates that mouse and rat are not recommended for IHC.

    For BDNF, multiple WB bands are possible and expected. The human protein has 5 isoforms (precursors: 28 – 37 kDa) and can be glycosylated (Uniprot: http://www.uniprot.org/uniprot/P23560). The mature form is expected at ~14 kDa (monomer) and the dimer at ~28 kDa.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.Can be blocked with Human BDNF peptide (ab182199).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/750.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
    • 組織特異性
      Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.
    • 関連疾患
      Bulimia nervosa 2
      Congenital central hypoventilation syndrome
    • 配列類似性
      Belongs to the NGF-beta family.
    • 翻訳後修飾
      The propeptide is N-glycosylated and glycosulfated.
      Converted into mature BDNF by plasmin (PLG).
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Abrineurin antibody
      • ANON2 antibody
      • BDNF antibody
      • BDNF_HUMAN antibody
      • Brain Derived Neurotrophic Factor antibody
      • Brain-derived neurotrophic factor antibody
      • BULN2 antibody
      • MGC34632 antibody
      • Neurotrophin antibody
      see all

    Anti-BDNF antibody [EPR1292] 画像

    • All lanes : Anti-BDNF antibody [EPR1292] (ab108319) at 1/1000 dilution

      Lane 1 : Human hippocampus lysate
      Lane 2 : Rat hippocampus lysate
      Lane 3 : Mouse hippocampus lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Gt anti Rb IR680 at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 15 kDa
      Additional bands at : 15 kDa (possible mature (processed) protein),28 kDa (possible multimer),35 kDa,45 kDa (possible immature (unprocessed)). We are unsure as to the identity of these extra bands.

      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab108319 (1/1000) overnight at 4°C. Ab8245 (mouse anti-GAPDH; 0.05 ug/mL) was included as a loading control. Antibody binding was detected using goat anti-rabbit IgG IR-680 (green) and goat anti-mouse IgG IR800 (red) at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx

    • Immunohistochemical analysis of brain tissue from sedentary (SED) or exercised rats (EX), staining BDNF with ab108319.

      Antigen retrieval was performed by enzymatic method at room temperature. Tissue was permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum. Samples were incubated with primary antibody (1/100) for 3 days at 3°C. An AlexaFluor®568-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody.
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108319 at 1/100
    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling BDNF with ab108319 at a dilution of 1/750. Cells were fixed with Paraformaldehyde and permeabilised with 0.5% Triton-X100 in PBS. ab150081 (1/200) was used as the secondary antibody.

      The antibody produces a strong, golgi-associated labelling pattern in both PF and MeOH fixed samples.

      See Abreview

    Anti-BDNF antibody [EPR1292] (ab108319) 使用論文

    This product has been referenced in:
    • Chen G  et al. Risperidone reverses the spatial object recognition impairment and hippocampal BDNF-TrkB signalling system alterations induced by acute MK-801 treatment. Biomed Rep 6:285-290 (2017). WB ; Rat . Read more (PubMed: 28451387) »
    • Monnier A  et al. Brain-derived neurotrophic factor of the cerebral microvasculature: a forgotten and nitric oxide-dependent contributor of brain-derived neurotrophic factor in the brain. Acta Physiol (Oxf) N/A:N/A (2016). Rat . Read more (PubMed: 27364224) »

    See all 16 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    15 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 23 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Endometrium (eutopic and ectopic))
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    Endometrium (eutopic and ectopic)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 May 31 2017

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    15 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    投稿 Apr 21 2017

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1
    Permeabilization
    No
    Specification
    cerebellum
    Fixative
    formalin
    Username

    Dr. Emmanuel Bouchaert

    Verified customer

    投稿 Oct 06 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Sep 29 2016

    Thank you for contacting us.
    We have quite a few antibodies for BDNF. The antibodies ab108319, ab108383 ab109049 or ab72439 are against mature BDNF however ab78367 would detect the pro form.
    I hope this information is helpful to you. Please d...

    Read More

    Thank you for contacting us and for your interest in our products. I think ab108319 (www.abcam.com/ab108319) or ab108383 (www.abcam.com/ab108383) would be suitable since you are looking for a monoclonal antibody. However, please note that none of t...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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