製品の概要

  • 製品名Anti-BCRP/ABCG2 antibody
    BCRP/ABCG2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BCRP/ABCG2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human, Pig
    交差が予測される動物種: Rat, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human BCRP/ABCG2.

    (Peptide available as ab100820.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Placenta Tissue Lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab63907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

ターゲット情報

  • 機能Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
  • 組織特異性Highly expressed in placenta. Low expression in small intestine, liver and colon.
  • 配列類似性Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
    Contains 1 ABC transmembrane type-2 domain.
    Contains 1 ABC transporter domain.
  • 翻訳後修飾Glycosylation-deficient ABCG2 is normally expressed and functional.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABC transporter antibody
    • ABC15 antibody
    • ABCG 2 antibody
    • ABCG2 antibody
    • ABCG2_HUMAN antibody
    • ABCP antibody
    • ATP binding cassette sub family G (WHITE) member 2 antibody
    • ATP binding cassette transporter G2 antibody
    • ATP-binding cassette sub-family G member 2 antibody
    • BCRP antibody
    • BCRP1 antibody
    • BMDP antibody
    • Breast cancer resistance protein antibody
    • CD338 antibody
    • CDw338 antibody
    • CDw338 antigen antibody
    • EST157481 antibody
    • GOUT1 antibody
    • MGC102821 antibody
    • Mitoxantrone resistance associated protein antibody
    • Mitoxantrone resistance-associated protein antibody
    • MRX antibody
    • Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2 antibody
    • MXR antibody
    • MXR1 antibody
    • Placenta specific ATP binding cassette transporter antibody
    • Placenta specific MDR protein antibody
    • Placenta-specific ATP-binding cassette transporter antibody
    • UAQTL1 antibody
    see all

Anti-BCRP/ABCG2 antibody 画像

  • Anti-BCRP/ABCG2 antibody (ab63907) at 1 µg/ml + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Secondary
    Secondary anti-rabbit antibody at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 10 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-BCRP/ABCG2 antibody (ab63907) 使用論文

This product has been referenced in:
  • Shapiro SD  et al. Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes. Sci Transl Med 6:224ra27 (2014). Read more (PubMed: 24553388) »
  • Barron GA  et al. Differential sensitivity in cell lines to photodynamic therapy in combination with ABCG2 inhibition. J Photochem Photobiol B 126:87-96 (2013). Read more (PubMed: 23911860) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 30 µg
Specification Kidney
Gel Running Conditions Reduced Non-Denaturing (Native) (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Kristin Bircsak

Verified customer

投稿 Feb 12 2013

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring prod...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Thank you for getting back to me promptly.

It is important to note that the actual (apparent) molecular weight values for the prestained markers can be different from the non-stained markers and also in different gel types.

When a p...

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Thank you for getting back to me and for sharing your results with me.

The storage buffer of the positive control lysate contains beta mercaptoethanol (reducing agent) so it is very unlikely that the protein is dimerized particularly if the W...

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Thank you for your e-mail.

I can confirm that the package has been delivered this morning 20Jul12 at 10:14 GMT and the person who signed for was: J. XXX.

Please check your Goods In Department. For your reference, tracking number was...

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Merci pour votre émail.

Oui, je peux confirmer que vous auriez droit à un autre anticorps aussi quand l'anticorps ne fonctionne pas.

Je peux aussi confirmer, que vous pouvez choisir unn'importe lequel de nos anticorps ...

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Ma collègue vient de me dire que vous voudriez faire le test chez le cochon - j'ai bien compris que vous voudriez donc tester le ab130244?

Voici ici aussi les informations promises et les détails de l'offre de test:

**...

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Thank you for your new contact.

As we discussed and agreed previously, I have placed the second free of charge replacement vial (ab29743) - exchange for the original product ab29745. The new order number is No: 1125597.

Currently, w...

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Just to confirm that you do not have to pay for the free of charge replacement vial. I have raised a credit note for the incorrect amount of £237.60 that has been charged. The reference number for the credit note is: CN21732

Apologies! ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"