The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5-1µg for 105-8 cells. A final staining volume of 100µl is recommended.
ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
機能Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.
組織特異性Highly expressed in placenta. Low expression in small intestine, liver and colon.
Staining of 3T3 cells transfected with Human BCRP/ABCG2 using 0.5µg of biotin-conjugated mouse IgG2b isotype control (open histogram) or 0.5 µg of ab95692 (filled histogram), followed by streptavidin PE. Total viable cells were used for analysis.