Anti-Bcr (phospho Y177) 抗体 [EPR576(2)Y] (ab68216)

製品の概要

  • 製品名Anti-Bcr (phospho Y177) antibody [EPR576(2)Y]
    Bcr 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR576(2)Y] to Bcr (phospho Y177)
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding tyrosine 177 of human Bcr.

  • ポジティブ・コントロール
    • 3T3 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab68216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000 - 1/2000. Detects a band of approximately 153 kDa (predicted molecular weight: 143 kDa).

    Is unsuitable for Flow Cyt, ICC, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
    • 関連疾患Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
    • 配列類似性Contains 1 C2 domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 Rho-GAP domain.
    • ドメインThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
      The DH domain is involved in interaction with CCPG1.
    • 翻訳後修飾Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ALL antibody
      • bcr antibody
      • BCR/ABL FUSION GENE, INCLUDED antibody
      • BCR/FGFR1 chimera protein antibody
      • BCR/FGFR1 FUSION GENE, INCLUDED antibody
      • BCR/PDGFRA FUSION GENE, INCLUDED antibody
      • BCR_HUMAN antibody
      • BCR1 antibody
      • Breakpoint cluster region antibody
      • Breakpoint cluster region protein antibody
      • CML antibody
      • D22S11 antibody
      • D22S662 antibody
      • FGFR1/BCR chimera protein antibody
      • PHL antibody
      • Renal carcinoma antigen NY-REN-26 antibody
      see all

    Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] 画像

    • All lanes : Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216) at 1/1000 dilution

      Lane 1 : 3T3 cell lysate, membrane un-treated
      Lane 2 : 3T3 cell lysate, membrane treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 143 kDa
      Observed band size : 153 kDa (why is the actual band size different from the predicted?)

    Anti-Bcr (phospho Y177) antibody [EPR576(2)Y] (ab68216) 使用論文

    ab68216 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68216.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"