製品の概要

  • 製品名Anti-Bcr antibody
    Bcr 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bcr
  • アプリケーション適用あり: WB, IP, Flow Cyt, ICC/IF, ICCmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Medaka fish, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1221 and 1271 of human Bcr using the numbering given in entry (NP_004318.3).

  • ポジティブ・コントロール
    • HeLa whole cell lysate 293T whole cell lysate K562 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 143 kDa.
IP Use at 10 µg/mg of lysate.
Flow Cyt Use 0.5µg for 106 cells. (in 150µl).



ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/500 - 1/2000.

Antigen retrieval with citrate buffer pH6.0 is recommended for formalin-fixed paraffin-embedded cells. For cytospin preparations of formaldehyde fixed cells permeabilization with Triton-X 100 is recommended.

ターゲット情報

  • 機能GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
  • 関連疾患Note=A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 配列類似性Contains 1 C2 domain.
    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 Rho-GAP domain.
  • ドメインThe region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.
    The DH domain is involved in interaction with CCPG1.
  • 翻訳後修飾Autophosphorylated. Phosphorylated by FES/FPS on tyrosine residues, leading to down-regulation of the BCR kinase activity. Phosphorylation at Tyr-177 by HCK is important for interaction with GRB2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALL antibody
    • bcr antibody
    • BCR/ABL FUSION GENE, INCLUDED antibody
    • BCR/FGFR1 chimera protein antibody
    • BCR/FGFR1 FUSION GENE, INCLUDED antibody
    • BCR/PDGFRA FUSION GENE, INCLUDED antibody
    • BCR_HUMAN antibody
    • BCR1 antibody
    • Breakpoint cluster region antibody
    • Breakpoint cluster region protein antibody
    • CML antibody
    • D22S11 antibody
    • D22S662 antibody
    • FGFR1/BCR chimera protein antibody
    • PHL antibody
    • Renal carcinoma antigen NY-REN-26 antibody
    see all

Anti-Bcr antibody 画像

  • Immunocytochemistry/Immunofluorescence analysis of human K562 cells labelling Bcr with ab86173 at 1/1000 (1µg/ml). An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
  • All lanes : Anti-Bcr antibody (ab86173) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 143 kDa


    Exposure time : 30 seconds
  • Detection of Human Bcr by Immunoprecipitation, using ab86173 at 10µg/mg lysate. Image shows immunoprecipitated Bcr detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg, with an antibody recognising an upstream epitope in lane 1, ab86173 at 1 µg/ml in lane 2 and control IgG in lane 3. Detection: Chemiluminescence with an exposure time of 10 seconds.
  • Flow Cytometric Detection of Bcr. 1 x 106 K562 cells were fixed, permeabilized, and stained with ab86173 at 0.25 µg in a 150 µl reaction. Image shows Isotype anti-KLH control (red), no antibody (blue) and ab86173 (black).
  • ICC/IF image of ab86173 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86173, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Bcr antibody (ab86173) 使用論文

ab86173 has not yet been referenced specifically in any publications.

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