Anti-BCMA 抗体 [Vicky-1] (ab17323)

製品の概要

  • 製品名Anti-BCMA antibody [Vicky-1]
    BCMA 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [Vicky-1] to BCMA
  • 特異性Recognizes human BCMA.
  • アプリケーション適用あり: ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Recombinant human BCMA:Fc. The extracellular domain of human BCMA (amino acids 2-54) is fused to the Fc portion of human IgG1.

  • ポジティブ・コントロール
    • U266 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC
Flow Cyt
  • 追加情報Flow Cyt: Use at a concentration of 1 µg/ml (see recommended protocol and figures).
    ICC: Use at a concentration of 5 µg/ml.
    Functional Application (blocks binding of BAFF to human BMCA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
    • 組織特異性Expressed in mature B-cells, but not in T-cells or monocytes.
    • 関連疾患Note=A chromosomal aberration involving TNFRSF17 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with IL2.
    • 配列類似性Contains 1 TNFR-Cys repeat.
    • 細胞内局在Cell membrane. Endomembrane system. Perinuclear Golgi-like structures.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B cell maturation antigen antibody
      • B cell maturation factor antibody
      • B cell maturation protein antibody
      • B-cell maturation protein antibody
      • BCM antibody
      • BCMA antibody
      • CD269 antibody
      • CD269 antigen antibody
      • TNFRSF17 antibody
      • TNR17_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 17 antibody
      see all

    Anti-BCMA antibody [Vicky-1] 画像

    • Immunostaining of HEK 293 cells transfected with a human BCMA expression plasmid (left panel), or mock transfected (right panel).
      Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone or 4% formaldehyde for 5min at RT. Slides were blocked with normal IgG, and incubated for 1 hour with 5µg/ml ab17323 in 1%BSA / 1x PBS. After washes in PBS, samples were incubated with the secondary antibody for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.

    • FACS analysis of cells with ab17323 to BCMA.

      Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for human BCMA. Cells (5x105) were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of ab17323 monoclonal antibody to human BCMA. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry.

    • Detection of endogenous human BCMA with ab17323 to BCMA (human)(Vicky-1) (second peak, to the right). Method: U266 cells (2x105) were incubated on ice for 30 minutes with 0.2 µg of ab17323 or an isotype control in 25µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide). The primary antibody was revealed with anti- Rat IgG (R-PE) and then analyzed by Flow Cytometry.

    Anti-BCMA antibody [Vicky-1] (ab17323) 使用論文

    ab17323 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I am sorry to confirm that as far as we are aware, ab17323 has never been tested with samples from cynomolgus monkey. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these a...

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    I'm sorry to hear the customer is experiencing problems in FACS with some of our antibodies, it is likely that the problem is protocol related. I have received this morning a detailed FACS protocol with details of the buffers to use and fixative to use...

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    The antibody to BCMA (Vicky-1) has been tested by FACS analysis on several cells (B cells from Myeloma U266 or in B cell Lymphoma suDHL-6). The endogenous protein is well detected. If you have any additional questions, please contact us again.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"