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Synthetic peptide (Human) - corresponding to residues in the C terminal region of Bcl6.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our Abpromise guarantee covers the use of ab33901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 85-90 kDa (predicted molecular weight: 78 kDa).|
|ICC||1/100 - 1/250.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19337254|
|IHC-P||Use at an assay dependent concentration. PubMed: 19337254|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human DLBCL cell line SUDHL6 xenograft labeling Bcl6 with ab33901 at 1/500 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA pH 9. 15% serum was used to block tissue for 1 hour at 20°C. A polyclonal Goat anti-Rabbit IgG biotin conjugated secondary antibody was used at 1/200 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"