Anti-Bcl3 抗体 [Ham150-3.5] (ab15046)
Key features and details
- Hamster monoclonal [Ham150-3.5] to Bcl3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bcl3 antibody [Ham150-3.5]
Bcl3 一次抗体 製品一覧 -
製品の詳細
Hamster monoclonal [Ham150-3.5] to Bcl3 -
由来種
Hamster -
特異性
ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms. -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Full length protein corresponding to Mouse Bcl3.
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ポジティブ・コントロール
- C57BI/6 human T cells and Bcl3 transgenic ms T cells. Flow Cyt: BAF3, Ramos cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
Ham150-3.5 -
アイソタイプ
IgG -
研究分野
関連製品
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15046の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
1 µg/test (100µl) |
特記事項 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa). |
Flow Cyt
Use at an assay dependent concentration. 1 µg/test (100µl) |
ターゲット情報
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機能
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
関連疾患
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
配列類似性
Contains 7 ANK repeats. -
翻訳後修飾
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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参照データベース
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
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別名
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
画像
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Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Flow cytometric analysis of 2% paraformaldehyde-fixed BAF3cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/ml
Predicted band size: 50 kDa
Western blot of Bcl3 in transgenic mouse T cells using Ab15046.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab15046 は論文での使用が確認できていません。