製品の概要

  • 製品名Anti-Bcl3 antibody
    Bcl3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bcl3
  • アプリケーション適用あり: WB, IP, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) - mapping to the C terminus of Bcl 3.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
IP Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 20 - 40 µg/ml.

ターゲット情報

  • 機能Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.
  • 関連疾患Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.
  • 配列類似性Contains 7 ANK repeats.
  • 翻訳後修飾Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
    Activated by phosphorylation.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B cell CLL/lymphoma 3 antibody
    • B cell leukemia/lymphoma 3 antibody
    • B cell lymphoma 3 encoded protein antibody
    • B-cell lymphoma 3 protein antibody
    • Bcl 3 antibody
    • BCL 4 antibody
    • BCL-3 antibody
    • Bcl3 antibody
    • BCL3_HUMAN antibody
    • BCL4 antibody
    • Chronic lymphatic leukemia protein antibody
    • D19S37 antibody
    • Protein Bcl 3 antibody
    • Proto-oncogene BCL3 antibody
    see all

Anti-Bcl3 antibody 画像

  • Anti-Bcl3 antibody (ab27780) at 2 µg/ml + Jurkat

    Predicted band size : 47 kDa

Anti-Bcl3 antibody (ab27780) 使用論文

ab27780 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Colon)
Loading amount 80 µg
Specification Colon
Treatment Basal and Knockdown (siRNA)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

投稿 Nov 23 2012

Thank you very much for that feedback.

I am pleased to hear that the anti-Bcl3 antibody ab49470 is showing promise in your experiments. This information would be very valuable to other customers so I would be very interested to hear when you ...

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That is no problem at all, we would be happy to receive the feedback whenever you are ready to give it.

I have now arranged for the two antibodies we discussed to be sent to you (free of charge). The antibody ab13708 is however not directly i...

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Thank you for getting back to me.

I can provide you any antibody which you would prefer. Ab13708 is marginally more popular but not by much. I will also check if we have any information on if the mouse monoclonal ab49470 is likely to work in ...

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Sorry for the delay in getting back to you.

I have checked the QC data for the particular lot of ab27780 you have been using and it looks as clean as the Western blot presented on the datasheet of this product using Jurkat cell lysate. One th...

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Thank you for calling us yesterday and for alerting us to the problem you are experiencing with theAnti-Bcl3 antibody (ab27780) and sorry for the delay in getting back to you. We take product complaints very seriously, and investigate every product tha...

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Thank you for your email.

I am pleased to hear that the ab49470 worked for you. I am currently seeking information as to whether we have a further 5 vials of the lot you tried in stock. I will get back to you with this information as soon as I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"