製品の概要

  • 製品名Anti-Bcl2L2 antibody
    Bcl2L2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bcl2L2
  • アプリケーション適用あり: IHC-FoFr, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl2L2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 21289171
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
  • 組織特異性Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインThe BH4 motif seems to be involved in the anti-apoptotic function.
    The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
  • 細胞内局在Mitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator BCL W antibody
    • Apoptosis regulator Bcl-W antibody
    • B2CL2_HUMAN antibody
    • BCL 2 Like 2 antibody
    • Bcl 2 like 2 protein antibody
    • Bcl 2L2 antibody
    • BCL W antibody
    • Bcl-2-like protein 2 antibody
    • Bcl2 L2 antibody
    • BCL2 like 2 antibody
    • BCL2 like 2 protein antibody
    • Bcl2-L-2 antibody
    • Bcl2l2 antibody
    • BCLW antibody
    • KIAA0271 antibody
    • PPP1R51 antibody
    • Protein phosphatase 1 regulatory subunit 51 antibody
    see all

Anti-Bcl2L2 antibody 画像

  • Anti-Bcl2L2 antibody (ab38629) + HL-60 cell lysate

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-Bcl2L2 antibody (ab38629) 使用論文

This product has been referenced in:
  • Ibrahim MY  et al. a-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther 8:1629-47 (2014). WB ; Human . Read more (PubMed: 25302018) »
  • Ibrahim MY  et al. Involvement of NF-?B and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, a-mangostin, from Cratoxylum arborescens. Drug Des Devel Ther 8:2193-211 (2014). WB ; Human . Read more (PubMed: 25395836) »

See all 3 Publications for this product

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