製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bcl2A1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    GST Fusion protein of human Bcl2A1 minus the transmembrane segment.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Tested on transfected cells and endogenous protein in various lympoid cell lines. 

IP Use 1-2µl for 106 cells.

 

ターゲット情報

  • 機能
    Retards apoptosis induced by IL-3 deprivation. May function in the response of hemopoietic cells to external signals and in maintaining endothelial survival during infection.
  • 組織特異性
    Seems to be restricted to the hematopoietic compartment. Expressed in peripheral blood, spleen, and bone marrow, at moderate levels in lung, small intestine and testis, at a minimal levels in other tissues. Also found in vascular smooth muscle cells and hematopoietic malignancies.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACC 1 antibody
    • ACC 2 antibody
    • B2LA1_HUMAN antibody
    • Bcl 2 related protein A1 antibody
    • Bcl-2-like protein 5 antibody
    • Bcl-2-related protein A1 antibody
    • BCL2 related protein A1 antibody
    • Bcl2-L-5 antibody
    • BCL2A1 antibody
    • BCL2L5 antibody
    • BFL1 antibody
    • GRS antibody
    • HBPA1 antibody
    • Hematopoietic BCL2 related protein A1 antibody
    • Hemopoietic specific early response protein antibody
    • Hemopoietic-specific early response protein antibody
    • Protein BFL 1 antibody
    • Protein BFL-1 antibody
    • Protein GRS antibody
    see all

画像



  • Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Western blot using ab75887 (1:1000)

    Lane 1: Invitro translated human Bcl2A1.

    Lane 2-5: Immunoprecipitates with:
    Lane 2: Ab75887 = anti-GST human Bcl2A1 (test)
    Lane 3: Normal rabbit serum
    Lane 4: Anti-HA monoclonal 12CA5
    Lane 5: Normal mouse serum
    Lane 6: Total lysate

    Lanes 7-10: Immunoprecipitates with:
    Lane 7: Ab75887
    Lane 8: Normal rabbit serum
    Lane 9: Anti-FLAG monoclonal M2
    Lane 10: Normal mouse serum
    Lane 11: Total lysate
  • Lanes 1-4: Immunoprecipitates with:

    Lane 1: Ab75887
    Lane 2: Normal rabbit serum
    Lane 3: Anti-HA monoclonal 12CA5
    Lane 4: Normal rabbit serum
    Lane 5: Total lysate

    Lanes 6-9: immunoprecipitates with:

    Lane 6: Ab75887
    Lane 7: Normal rabbit serum
    Lane 8: Anti-FLAG monoclonal M2
    Lane 9: Normal mouse serum
    Lane 10: Total lysate.


  • Predicted band size : 20 kDa
    Ab75887 was tested on human B cell lines by Dr Natalie Bonnefoy-Berard and colleagues.

    Detection of endogenous human Bcl2A1 in total lysates of B cell lines and in transfected 3T3 and 293T cells by ab75887.

    Expression in B cell lines as detected by ab75887 correlates with mRNA levels as detected by RT-PCR, further confirming the specificity of the antibody.

参考文献

This product has been referenced in:
  • Chien WW  et al. Differential mechanisms of asparaginase resistance in B-type acute lymphoblastic leukemia and malignant natural killer cell lines. Sci Rep 5:8068 (2015). WB ; Human . Read more (PubMed: 25626693) »
  • Olsson Åkefeldt S  et al. Chemoresistance of human monocyte-derived dendritic cells is regulated by IL-17A. PLoS One 8:e56865 (2013). WB ; Human . Read more (PubMed: 23441221) »

See all 3 Publications for this product

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