製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bcl-XL
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Cow, Cat, Pig
  • 免疫原

    Recombinant fragment corresponding to Human Bcl-XL aa 1-178.
    Database link: Q07817

  • ポジティブ・コントロール
    • A549, HepG2, HCT116, NIH 3T3 cell lysates; SW480 xenograft This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa and HepG2.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab98143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 組織特異性
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • ドメイン
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻訳後修飾
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 細胞内局在
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

画像

  • ICC/IF image of ab98143 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98143 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%, 10min) hepG2 cells at 5ug/ml.

  • Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Anti-Bcl-XL antibody (ab98143) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of Bcl x in paraffin embedded SW480 xenograft, using ab98143 at a 1/500 dilution.

参考文献

This product has been referenced in:
  • Grammas P  et al. Brain microvasculature and hypoxia-related proteins in Alzheimer's disease. Int J Clin Exp Pathol 4:616-27 (2011). WB ; Rat . Read more (PubMed: 21904637) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Nov 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate)
Specification
prostate
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 100mM sodium cytrate
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Hadas Pahima

Verified customer

投稿 Oct 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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