製品の概要

  • 製品名Anti-Bcl-XL antibody
    Bcl-XL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bcl-XL
  • 特異性This antibody detects the 30 kDa Bcl-XL protein. It does not react with other Bcl-2 family members.
  • アプリケーション適用あり: IP, WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Bcl-XL. Synthetic peptide surrounding amino acid 56 of human Bcl-xL
    Database link: Q07817
    (Peptide available as ab52114)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2568 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 20 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.Can be blocked with Human Bcl-XL peptide (ab52114).
ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 組織特異性Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 配列類似性Belongs to the Bcl-2 family.
  • ドメインThe BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻訳後修飾Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 細胞内局在Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody 画像

  • ICC/IF image of ab2568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab2568 staining BCL-XL (green) in Mouse RAW 264.7 cells by ICC (Immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized by 0.1% Triton X-100 in 2% BSA for 15 minutes and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/250 in 2% BSA in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Chicken anti-rabbit IgG polyclonal (1/750) was used as the secondary antibody.
    WGA (red) = Alexa Fluor®568-conjugated Wheat Germ Agglutinin

    See Abreview

  • Anti-Bcl-XL antibody (ab2568) at 1/1000 dilution + 25 ug HeLa whole cell lysate at 25 µg

    Secondary
    Goat anti rabbit (HRP) at 1/4000 dilution

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    This image was submitted as part of an anonymous Abreview received on the 30th September 2005. We do not have any additional information relating to this image.

    See Abreview

  • All lanes : Anti-Bcl-XL antibody (ab2568) at 1/1000 dilution

    Lane 1 : 20ug whole cell lysate from Retinal Pigment Epithelia
    Lane 2 : 20ug whole cell lysate from MDA MB231 cells
    Lane 3 : 20ug whole cell lysate from HeLa cells

    Secondary
    Goat anti-rabbit HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
        This image is courtesy of an Abreview submitted by Ingram Iaccarino.

    See Abreview

  •     ab2568 used to immunoprecipitate Bcl xL from whole cell lysate of a human Retinal Pigment Epithelial cell line. 7.5ug of antibody was added per mg of lysate and incubated together with Protein A for 1 hour. 500ug of lysate was added per lane. A peroxidase conjugated goat anti-rabbit IgG was used as the secondary antibody.

    Lane 1: Immunoprecipitated material

    Lane 2:  Control lane (no antibody)

    This image is courtesy of an Abreview submitted by Ingram Iaccarino on 12 April 2006

    See Abreview

Anti-Bcl-XL antibody (ab2568) 使用論文

This product has been referenced in:
  • Shokhirev MN  et al. A multi-scale approach reveals that NF-?B cRel enforces a B-cell decision to divide. Mol Syst Biol 11:783 (2015). ICC/IF ; Mouse . Read more (PubMed: 25680807) »
  • Fresquet V  et al. Acquired mutations in BCL2 family proteins conferring resistance to the BH3 mimetic ABT-199 in lymphoma. Blood 123:4111-9 (2014). WB ; Human . Read more (PubMed: 24786774) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (RAW 264.7)
Specification RAW 264.7
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 May 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment Calcium Ionophore for 1 h
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Nov 14 2011

Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

投稿 Dec 22 2010

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. We have received some excellent feedback for this antibody in the form of customer "Abreviews". They all detail a strong band of approximately ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela and 293T cells)
Loading amount 10 µg
Specification Hela and 293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 14 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Retinal Pigment Epithelial cell line)
Total protein in input 500 µg
Specification Retinal Pigment Epithelial cell line
Immuno-precipitation step Protein A
Username

Dr. Ingram Iaccarino

Verified customer

投稿 Apr 27 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (RPE, MDA MB231, HeLa)
Loading amount 20 µg
Specification RPE, MDA MB231, HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ingram Iaccarino

Verified customer

投稿 Apr 13 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Sep 30 2005

Thank you for your enquiry. All the information that we have regarding these two antibodies is located on the online datasheets, including references that we are currently aware of. If you have any more questions please contact us again.

Ab2568 is against a peptide. However, ab2568 actually utilizes the whole protein as immunogen.

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"