製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bcl-XL
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-XL aa 46-66.
    Sequence:

    ETPSAINGNPSWHLADSPAVN


    Database link: Q07817

  • ポジティブ・コントロール
    • Hodgkins lymphoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P 1/200. for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes.

ターゲット情報

  • 機能
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 組織特異性
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • ドメイン
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻訳後修飾
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 細胞内局在
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

画像

  • Anti-Bcl-XL antibody (ab15274) at 1/25 dilution + 293 cell lysate

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
    Additional bands at : 45 kDa,52 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Zhang K  et al. Bcl-xL overexpression and its association with the progress of tongue carcinoma. Int J Clin Exp Pathol 7:7360-77 (2014). Read more (PubMed: 25550772) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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