Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)
製品の概要
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製品名
Bcl G/BCL2L14 overexpression 293T lysate (whole cell) -
特記事項
ab94328 is a 293T cell transfected lysate in which Human Bcl G/BCL2L14 has been transiently over-expressed using a pCMV-Bcl G/BCL2L14 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94328の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94328 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Bcl G/BCL2L14 antibody (ab57742) at 1/500 dilution
Lane 1 :Bcl G/BCL2L14 overexpression 293T lysate (whole cell) (ab94328)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94328 は論文での使用が確認できていません。