製品の概要

  • 製品名
    Anti-Bcl-2 (phospho S70) antibody
    Bcl-2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bcl-2 (phospho S70)
  • 特異性
    ab28819 recognises Phospho-BCL-2(Ser70).
  • アプリケーション
    適用あり: WB, ELISA, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-2.
    Database link: P10415

  • ポジティブ・コントロール
    • Breast carcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration. PubMed: 19738029ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • 組織特異性
    Expressed in a variety of tissues.
  • 関連疾患
    A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • 配列類似性
    Belongs to the Bcl-2 family.
  • ドメイン
    BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • 翻訳後修飾
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • 細胞内局在
    Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptosis regulator Bcl 2 antibody
    • Apoptosis regulator Bcl-2 antibody
    • Apoptosis regulator Bcl2 antibody
    • AW986256 antibody
    • B cell CLL/lymphoma 2 antibody
    • B cell leukemia/lymphoma 2 antibody
    • Bcl-2 antibody
    • Bcl2 antibody
    • BCL2_HUMAN antibody
    • C430015F12Rik antibody
    • D630044D05Rik antibody
    • D830018M01Rik antibody
    • Leukemia/lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    • PPP1R50 antibody
    • Protein phosphatase 1, regulatory subunit 50 antibody
    see all

画像



  • Predicted band size : 26 kDa

    Western blot analysis of lysates from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the phospho peptide.

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma, using anti-Phospho-BCL2(Ser70) antibody (ab28819). Left: Untreated; Right: Treated with synthesized phosphopeptide.

参考文献

This product has been referenced in:
  • Fan L  et al. Salicylate •Phenanthroline copper (II) complex induces apoptosis in triple-negative breast cancer cells. Oncotarget 8:29823-29832 (2017). IHC . Read more (PubMed: 28415735) »
  • Kendrick SL  et al. BCL2 antibodies targeted at different epitopes detect varying levels of protein expression and correlate with frequent gene amplification in diffuse large B-cell lymphoma. Hum Pathol 45:2144-53 (2014). Read more (PubMed: 25090918) »

See all 3 Publications for this product

レビューと Q&A

I was unable to find out the exact immunogen sequence used to generate ab28819 unfortunately, however a homology search between the whole human protein and the rat protein revealed a 90% homology, so the antibody has some good chances of working in rat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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