製品の概要

  • 製品名Anti-BCAS3 antibody
    BCAS3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BCAS3
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rhesus monkey, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)

  • ポジティブ・コントロール
    • Whole cell lysate from MCF7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
  • 関連疾患Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
  • 配列類似性Belongs to the WD repeat BCAS3 family.
    Contains 3 WD repeats.
  • 発生段階Fetal.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1500019F07Rik antibody
    • 2610028P08Rik antibody
    • AU021018 antibody
    • BC028339 antibody
    • BCAS3 antibody
    • BCAS3_HUMAN antibody
    • Breast carcinoma-amplified sequence 3 antibody
    • FLJ20128 antibody
    • GAOB1 antibody
    • K20D4 antibody
    • MAAB antibody
    • Maab1 protein antibody
    • MGC4973 antibody
    • rudhira antibody
    see all

Anti-BCAS3 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
    Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
    Lane 3 : Whole cell lysate from MCF7 cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
    Lane 1: ab71162 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 1 second.

Anti-BCAS3 antibody (ab71162) 使用論文

ab71162 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Melanoma Cell Line)
Total protein in input 1000 µg
Immuno-precipitation step Protein A
Specification Melanoma Cell Line
Username

Mr. John Finnigan

Verified customer

投稿 Apr 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"