製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BCAR3
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 25 and 75 of human BCAR3 (NP_003558.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab85940 was affinity purified using an epitope specific to BCAR3 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2 - 5 µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 93 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
    • 組織特異性
      Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.
    • 配列類似性
      Contains 1 Ras-GEF domain.
      Contains 1 SH2 domain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BCAR 3 antibody
      • BCAR3 antibody
      • BCAR3_HUMAN antibody
      • Breast cancer anti estrogen resistance 3 antibody
      • Breast cancer anti estrogen resistance protein 3 antibody
      • Breast cancer anti-estrogen resistance protein 3 antibody
      • Breast cancer antiestrogen resistance 3 antibody
      • dJ1033H22.2 antibody
      • dJ1033H22.2 breast cancer anti estrogen resistance 3 antibody
      • dJ1033H22.2 breast cancer antiestrogen resistance 3 antibody
      • KIAA0554 antibody
      • Novel SH2 containing protein 2 antibody
      • Novel SH2-containing protein 2 antibody
      • NSP 2 antibody
      • NSP2 antibody
      • SH2 containing protein Nsp2 antibody
      • SH2 domain containing protein 3B antibody
      • SH2 domain-containing protein 3B antibody
      • SH2D3B antibody
      see all

    画像

    • All lanes : Anti-BCAR3 antibody (ab85940) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 93 kDa
      Observed band size : 93 kDa
      Additional bands at : 300 kDa,460 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 3 minutes
    • Detection of human BCAR3 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85940 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    参考文献

    ab85940 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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